Nine fluorescence parameter analysis on a four-color fluorescence activated flow cytometer
✍ Scribed by Ursula Frischmann; Werner Müller
- Publisher
- John Wiley and Sons
- Year
- 2006
- Tongue
- English
- Weight
- 303 KB
- Volume
- 69A
- Category
- Article
- ISSN
- 0196-4763
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✦ Synopsis
Abstract
Background
In many cases a frequent monitoring of blood is important, e.g. during an infection. Often the availability of blood is the limiting factor.
Methods
50 μl blood were stained and analyzed using a standard four‐color cytometer. Percentages of leukocytes were calculated by FlowJo software.
Results
Our protocol allows the differentiation of B cells, T cells, NK cells, neutrophils, and monocytes/macrophages in small volumes of blood.
Conclusions
Using nine fluorochrome‐labeled antibodies and a specific gating strategy we were able to differentiate the main immune cells in minute amounts using one staining step. © 2006 International Society for Analytical Cytology