Nicking Activity of TrwC Directed Against the Origin of Transfer of the IncW Plasmid R388
β Scribed by Matxalen Llosa; Guadalupe Grandoso; Fernando de la Cruz
- Book ID
- 115626353
- Publisher
- Elsevier Science
- Year
- 1995
- Tongue
- English
- Weight
- 549 KB
- Volume
- 246
- Category
- Article
- ISSN
- 0022-2836
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The broad jost range plasmid, RK2, has been isolated as a DNA-protein relaxation complex. Nicking of the plasmid DNA in the relaxation complex occurs at a single specific site (rlx) located approximately 20 kb away from the origin of DNA replication. A cis-acting function required for plasmid transf
The origin of transfer (oriT) is the sequence within which conjugal transfer of plasmid DNA is initiated, and is absolutely required in cis for plasmid mobilization. We have cloned oriT from the 52 kb IncN plasmid R46 on a 600 bp fragment, and mapped the limits of the relevant sequence by deletion a