Nickel hydroxy carbonate increases tumour necrosis factor alpha and interleukin 6 secretion by alveolar macrophages

✍ Scribed by Karim Arsalane; Philippe Gosset; Harmut F. Hildebrand; Cyr Voisin; André-Bernard Tonnel; Benoit Wallaert

• Publisher: John Wiley and Sons
• Year: 1994
• Tongue: English
• Weight: 498 KB
• Volume: 14
• Category: Article
• ISSN: 0260-437X
• DOI: 10.1002/jat.2550140510

✦ Synopsis

The aim of the current study was to assess the in vitro effects of nickel hydroxy carbonate (NiHC) at noncytotoxic concentrations on the production of cytokines such as tumour necrosis factor alpha (TNF-a) and interleukin 6 (IL-6) in alveolar macrophages (AMs). The effect of NiHC was evaluated in both unstimulated AMs and cells activated by lipopolysaccharide (LPS). Cytotoxicity was related to lactate dehydrogenase release and ATP cell content. The results confirm that NiHC at concentrations of 0.125, 1.25 and 3.125 p g NiHC cells was not cytotoxic. The NiHC exposure of unstimulated AMs significantly increased the release of TNF-a at all concentrations and that of IL-6 at 1.25 p g NiHC cells. LPS addition significantly increased the secretion of both cytokines. However, NiHC did not cause a significant increase in the release of TNF-a and IL-6 in LPS-stimulated cells. In conclusion, the ability of NiHC to activate AMs and to release increased amounts of pro-inflammatory mediators may be responsible, at least partly, for inflammation and pneumotoxicity associated with nickel exposure.

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MATERIAL AND METHODS

Material

Nickel hydroxy carbonate as the ALFA product 89019 (NiC03.xH20) was provided by the INCO J. Roy