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Next generation phage display by use of pVII and pIX as display scaffolds

✍ Scribed by Geir Åge Løset; Inger Sandlie


Book ID
118507077
Publisher
Elsevier Science
Year
2012
Tongue
English
Weight
387 KB
Volume
58
Category
Article
ISSN
1046-2023

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The metallo-b-lactamase bLII from Bacillus cereus 569/H/9 was displayed on the filamentous phage fd. The phage-bound enzyme fd-bLII was shown to be active on benzylpenicillin as substrate; it could be inactivated by complexation of the essential zinc(II ) ion with EDTA and reactivated by addition of