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New Techniques for the Measurement of C′N and C′HNJ Coupling Constants across Hydrogen Bonds in Proteins

✍ Scribed by Axel Meissner; Ole Winneche Sørensen

Publisher
Elsevier Science
Year
2000
Tongue
English
Weight
75 KB
Volume
143
Category
Article
ISSN
1090-7807

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✦ Synopsis

Two new two- or three-dimensional NMR methods for measuring (3h)J(C'N) and (2h)J(C'H) coupling constants across hydrogen bonds in proteins are presented. They are tailored to suit the size of the TROSY effect, i.e., the degree of interference between dipolar and chemical shift anisotropy relaxation mechanisms. The methods edit 2D or 3D spectra into two separate subspectra corresponding to the two possible spin states of the (1)H(N) spin during evolution of (13)CO coherences. This allows (2h)J(C'H) to be measured in an E.COSY-type way while (3h)J(C'N) can be measured in the so-called quantitative way provided a reference spectrum is also recorded. A demonstration of the new methods is shown for the (15)N,(13)C-labeled protein chymotrypsin inhibitor 2.

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