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New shuttle vectors for Rhodococcus sp. R312 (formerly Brevibacterium sp. R312), a nitrile hydratase producing strain

✍ Scribed by Robert Duran


Publisher
John Wiley and Sons
Year
1998
Tongue
English
Weight
100 KB
Volume
38
Category
Article
ISSN
0233-111X

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Purification and properties of the β-glu
✍ J. L. Legras; M. R. Kaakeh; Dr. A. Arnaud; P. Galzy 📂 Article 📅 1989 🏛 John Wiley and Sons 🌐 English ⚖ 555 KB

Besides its nitrile hydratase and wide spectrum amidase activities, the Brevibacterium sp. R312 strain also possesses a constitutive beta-glucosidase. Its optimum pH is 6. The enzyme was purified by fractionation precipitation with ammonium sulfate followed by chromatographic elutions on Q-Sepharose