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New rapid gel sequencing method for RNA

✍ Scribed by SIMONCSITS, A.; BROWNLEE, G. G.; BROWN, R. S.; RUBIN, J. R.; GUILLEY, H.


Book ID
109707521
Publisher
Nature Publishing Group
Year
1977
Tongue
English
Weight
785 KB
Volume
269
Category
Article
ISSN
0028-0836

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A simple and rapid solid-phase RNA seque
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A simple and rapid solid-phase RNA sequencing method has been developed based on Peattie's direct chemical method. 3'-Terminally labeled RNA was immobilized on DEAE-cellulose sheets and followed by specific modification with dimethyl sulfate, diethylpyrocarbonate, hydroxylamine (at pH 10 for the uri