New procedures for rapid screening of leaf lipid components from Arabidopsis

✍ Scribed by William W. Christie; Samantha Gill; Johan Nordbäck; Yutaka Itabashi; Sherrie Sanda; Antoni R. Slabas

• Publisher: John Wiley and Sons
• Year: 1998
• Tongue: English
• Weight: 104 KB
• Volume: 9
• Category: Article
• ISSN: 0958-0344
• DOI: 10.1002/(sici)1099-1565(199803/04)9:2<53::aid-pca391>3.0.co;2-u

✦ Synopsis

Four procedures have ben developed to simplify routine analysis of large numbers of plant lipid samples. First, a rapid extraction method has been devised in which the key step consists of washing propan-2-olchloroform extracts with an aqueous phase impregnating solid Sephadex TM G-25-300. The extracts are then separated by high performance liquid chromatography (HPLC) with evaporative light-scattering detection, using a complex gradient that permits separation of most of the important simple lipid, glycolipid and phospholipid classes in sequence. An example of an application to the analysis of a newly generated mutant is illustrated. In addition, a novel solid-phase extraction procedure with a bonded propylamine phase allows separation of four broad classes, simple lipids, glycolipids, zwitterionic phospholipids and acidic phospholipids (plus sulphoquinovosyldiacylglycerol), for further analysis. Finally, base-catalysed transesterification followed by HPLC analysis, permits identification of those lipids that do not contain conventional ester bonds.