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New PCR-based methods for yeast identification

✍ Scribed by N. Hierro; Á. González; A. Mas; J.M. Guillamón

Book ID
108837715
Publisher
John Wiley and Sons
Year
2004
Tongue
English
Weight
147 KB
Volume
97
Category
Article
ISSN
1364-5072

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Gene disruption and tagging can be achieved by homologous recombination in the yeast genome. Several PCR-based methods have been described towards this end. However these strategies are often limited in their applications and/or their efficiencies and may be technically demanding. Here we describe t