New monoclonal antibodies to oestrogen and progesterone receptors effective for paraffin section immunohistochemistry
✍ Scribed by Bevitt, Debra J.; Milton, Ian D.; Piggot, Nigel; Henry, Linda; Carter, Michael J.; Toms, Geoffrey L.; Lennard, Tom W. J.; Westley, Bruce; Angus, Brian; Horne, Charles H. W.
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- English
- Weight
- 348 KB
- Volume
- 183
- Category
- Article
- ISSN
- 0022-3417
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✦ Synopsis
Assessment of oestrogen and progesterone receptors (ER and
PgR) in breast cancer is widely used for the prediction of response to endocrine therapy and as a prognostic marker. Cytosolic assays have been replaced in many centres by immunochemical techniques, which have many advantages including applicability to small samples, simplicity, and cost-effectiveness. This study describes the generation and characterisation of two novel murine monoclonal antibodies recognizing ER and PgR, designated NCL-ER-6F11 and NCL-PGR respectively, which are effective in heat-treated formalin-fixed, paraffin-embedded tissue. The antibodies have been characterized by Western blotting and by immunohistochemistry on normal and pathological breast and other tissues. NCL-ER-6F11 has been shown to compare favourably with a currently available ER antibody. These antibodies may prove of value in the assessment of hormone receptor status in human breast cancer. 1997 John Wiley & Sons, Ltd.