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New methods for binding enzyme molecules into a water-insoluble matrix: Properties after insolubilization

✍ Scribed by G. Broun; D. Thomas; G. Gellf; D. Domurado; A. M. Berjonneau; C. Guillon


Publisher
John Wiley and Sons
Year
1973
Tongue
English
Weight
710 KB
Volume
15
Category
Article
ISSN
0006-3592

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✦ Synopsis


Abstract

New methods of crosslinking enzyme molecules inside a matrix with or without an inactive protein are described. Enzyme activity yields range between 30 and 80% of the activity of the untreated preparations. Even fragile enzyme systems, for instance those using mobile cofactors, can be efficiently immobilized. Increased resistance towards heat denaturation and proteolysis results.