New method for peptide desorption from abundant blood proteins for plasma/serum peptidome analyses by mass spectrometry
✍ Scribed by Rustam Ziganshin; Georgii Arapidi; Igor Azarkin; Elena Zaryadieva; Dmitry Alexeev; Vadim Govorun; Vadim Ivanov
- Book ID
- 104027648
- Publisher
- Elsevier
- Year
- 2011
- Tongue
- English
- Weight
- 989 KB
- Volume
- 74
- Category
- Article
- ISSN
- 1874-3919
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✦ Synopsis
This report describes a new method for desorption of low-molecular weight (LMW) peptides from abundant blood proteins for use in subsequent mass spectrometry analyses. Heating of diluted blood serum to 98 °C for 15 min resulted in dissociation of LMW peptides from the most abundant blood proteins. Application of blood plasma/serum fractionation using magnetic beads with a functionalized surface followed by heating of the resultant fractions significantly increases the number of LMW peptides detected by MALDI-TOF MS, enhances the general reproducibility of mass spectrometry profiles and considerably increases the number of identified blood serum peptides by LC-MS/MS using an Agilent 6520 Accurate-Mass Q-TOF.