𝔖 Bobbio Scriptorium
✦   LIBER   ✦

New member of aldose reductase family proteins overexpressed in human hepatocellular carcinoma

✍ Scribed by Zorica Scuric; Steven C. Stain; W. French Anderson; Jung-Joo Hwang

Publisher
John Wiley and Sons
Year
1998
Tongue
English
Weight
159 KB
Volume
27
Category
Article
ISSN
0270-9139

No coin nor oath required. For personal study only.

✦ Synopsis

The multistep process of liver carcinogenesis involves various genetic and phenotypic alterations. To identify genes whose expression is increased during hepatocarcinogenesis, differential-display polymerase chain reaction (DD-PCR) was used to examine differences in the mRNA composition of hepatocellular carcinoma (HCC) versus normal liver (nontumor) tissues. This approach identified 67 cDNAs that were preferentially expressed in HCC tissue. When these cDNAs were analyzed by reverse-Northern analysis, five were reproducibly expressed at high levels in HCC. Interestingly, Northern blot analysis revealed that one of the genes showed significantly increased mRNA levels in all five tested tumor samples, while its mRNA level in the nontumor samples was minimal. BLAST analysis revealed that this gene has high sequence identity with the genes from aldo-keto reductase family of proteins including the mouse fibroblast growth factor-induced gene (FR-1) (80% identity), mouse vas deferens protein (MVDP) (76%), and human aldose reductase (AR) (62%). Expression of this novel AR-related protein in all five tested HCCs suggests that this protein may play an important role in liver carcinogenesis.

📜 SIMILAR VOLUMES

Reg IV, a new member of the regenerating
Reg IV, a new member of the regenerating gene family, is overexpressed in colorectal carcinomas
✍ Sabine Violette; Estelle Festor; Ivona Pandrea-Vasile; Valérie Mitchell; Colette 📂 Article 📅 2002 🏛 John Wiley and Sons 🌐 French ⚖ 683 KB

## Abstract A better understanding of the mechanisms by which colon tumor cells are able to survive exposure to drugs would be valuable for the development of new therapeutic strategies. We used differential display‐PCR to compare gene expression in the drug‐sensitive HT‐29 colon cancer cell line a

Expression and localization of human mul
Expression and localization of human multidrug resistance protein (ABCC) family members in pancreatic carcinoma
✍ Jörg König; Mark Hartel; Anne T. Nies; Marc E. Martignoni; Junchao Guo; Markus W 📂 Article 📅 2005 🏛 John Wiley and Sons 🌐 French ⚖ 412 KB

## Abstract Pancreatic ductal adenocarcinoma is among the top 10 causes of death from cancer in industrialized countries. In comparison with other gastrointestinal malignancies, pancreatic cancer is one of the tumors most resistant to chemotherapy. An important mechanism of tumor multidrug resistan

Protein expression and amplification of
Protein expression and amplification of AIB1 in human urothelial carcinoma of the bladder and overexpression of AIB1 is a new independent prognostic marker of patient survival
✍ Jun-Hang Luo; Dan Xie; Meng-Zhong Liu; Wei Chen; Yong-Dong Liu; Guo-Qing Wu; Hsi 📂 Article 📅 2008 🏛 John Wiley and Sons 🌐 French ⚖ 342 KB 👁 1 views

## Abstract __AIB1__, a candidate oncogene in human breast cancer, is frequently amplified and overexpressed in several types of human cancers, but the status of __AIB1__ amplification and expression in urothelial carcinoma of the bladder (UC) and its clinical/prognostic significance is unclear. In