✦ LIBER ✦
New knowledge about thePHA-locus and P(3HB) granule-associated proteins inChromatium vinosum
✍ Scribed by Matthias Liebergesell; Alexander Steinbüchel
- Publisher
- Springer Netherlands
- Year
- 1996
- Tongue
- English
- Weight
- 462 KB
- Volume
- 18
- Category
- Article
- ISSN
- 0141-5492
No coin nor oath required. For personal study only.
✦ Synopsis
The isolation of poly(3-hydroxybutyric acid) granules of Chromatium vinosum D was re-examined. Beside the PHA synthase and a 17 kDa protein, a 14 kDa protein was identified as predominant granule-associated protein. The Mr as well as the N-terminal amino acid sequence exhibited identity to ORF5Cv, which is located within the pha-locus of C. vinosum. In addition, sequence alignements revealed new information about ORF4cv, which is also located within the pha-locus, and about the 17 kDa protein, which exhibited homology to heat shock proteins recently detected in Escherichia coli.