New infectious mammary tumor virus superantigen with Vβ-specificity identical to staphylococcal enterotoxin B (SEB)
✍ Scribed by Sanjiv Lutheru; Alexander N. Shakhov; Ioannis Xenariosu; Satomi Hagau; Shunsuke Imaiu; Hans Acha-Orbea
- Book ID
- 102824335
- Publisher
- John Wiley and Sons
- Year
- 1994
- Tongue
- English
- Weight
- 877 KB
- Volume
- 24
- Category
- Article
- ISSN
- 0014-2980
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✦ Synopsis
New infectious mammary tumor virus superantigen with Vp-specificity identical to staphylococcal enterotoxin B (SEB)
Only few infectious mouse mammary tumor viruses (MMTV) have been characterized which induce a potent superantigen response in vivo. Here we describe the characterization of an MMTV which was isolated from milk of the highly mammary tumor-prone SHN mouse strain. Exposure of newborn mice to milk-borne MMTV (SHN) results in a very slow deletion of y37,8.1,8.2 and 8.3 expressing peripheral T cells. Subcutaneous injection of adult mice with this virus induces a rapid and strong stimulation of all four affected Vp-subsets in vivo.
Besides the strongT cell effect we observed an early proliferation and activation of the local B cell pool leading to the initial secretion of IgM followed by preferential secretion of IgG2, by day 6. Sequence comparison of the polymorphic C terminus with known open reading frames revealed high homology to the endogenous provirus Mtv-RCS.This is the first report of a virus having a complete overlap inVp-specificity with a bacterial superantigen stimulating as many as 35 YO of the whole CD4+ T cell repertoire including Vp8.2. Abbreviations: M M W . Mouse mammary tumor virus Mtv: Mammary tumor virus locus MIS: Minor lymphocyte stimulating antigen orf: Open reading frame Vb: Variable region of theTcR 0 chain