New High Throughput Technologies for DNA Sequencing and Genomics

✍ Scribed by Keith R. Mitchelson (Eds.)

• Publisher: Elsevier Science
• Year: 2007
• Tongue: English
• Leaves: 399
• Series: Perspectives in Bioanalysis 2
• Edition: 1
• Category: Library

✦ Synopsis

Since the independent invention of DNA sequencing by Sanger and by Gilbert 30 years ago, it has grown from a small scale technique capable of reading several kilobase-pair of sequence per day into today's multibillion dollar industry. This growth has spurred the development of new sequencing technologies that do not involve either electrophoresis or Sanger sequencing chemistries. Sequencing by Synthesis (SBS) involves multiple parallel micro-sequencing addition events occurring on a surface, where data from each round is detected by imaging. New High Throughput Technologies for DNA Sequencing and Genomics is the second volume in the Perspectives in Bioanalysis series, which looks at the electroanalytical chemistry of nucleic acids and proteins, development of electrochemical sensors and their application in biomedicine and in the new fields of genomics and proteomics. The authors have expertly formatted the information for a wide variety of readers, including new developments that will inspire students and young scientists to create new tools for science and medicine in the 21st century. Reviews of complementary developments in Sanger and SBS sequencing chemistries, capillary electrophoresis and microdevice integration, MS sequencing and applications set the framework for the book. * 'Hot Topic' with DNA sequencing continuing as a major research activity in many areas of life science and medicine. Bringing together new developments in DNA sequencing technology Reviewing issues relevant to the new applications used

✦ Table of Contents

Content:
Contributors
Pages xi-xiii

Preface
Page xv

Chapter 1 Overview: Developments in DNA Sequencing Review Article
Pages 3-44
Keith R. Mitchelson, David B. Hawkes, Rustam Turakulov, Artem E. Men

Chapter 2 Chip Capillary Electrophoresis and Total Genetic Analysis Systems Review Article
Pages 45-95
Qiang Xiong, Jing Cheng

Chapter 3 Comparative Sequence Analysis by MALDI-TOF Mass Spectrometry – Utilizing the Known to Discover the New Review Article
Pages 97-117
Mathias Ehrich, Franz Hillenkamp, Dirk van den Boom

Chapter 4 Advances in Dye-Nucleotide Conjugate Chemistry for DNA Sequencing Review Article
Pages 119-149
Shiv Kumar, Carl W. Fuller

Chapter 5 The 454 Life Sciences Picoliter Sequencing System Review Article
Pages 153-186
Marcel Margulies, Thomas P. Jarvie, James R. Knight, Jan Fredrik Simons

Chapter 6 An Integrated System for DNA Sequencing by Synthesis Review Article
Pages 187-205
John R. Edwards, Dae Hyun Kim, Jingyue Ju

Chapter 7 Single-Molecule Fluorescence Microscopy and its Applications to Single-Molecule Sequencing by Cyclic Synthesis Review Article
Pages 209-244
Benedict Hebert, Ido Braslavsky

Chapter 8 Rapid DNA Sequencing by Direct Nanoscale Reading of Nucleotide Bases on Individual DNA chains Review Article
Pages 245-263
James Weifu Lee, Amit Meller

Chapter 9 A Single Molecule System for Whole Genome Analysis Review Article
Pages 265-300
Shiguo Zhou, Jill Herschleb, David C. Schwartz

Chapter 10 Sequencing Aided by Mutagenesis Facilitates the De Novo Sequencing of Megabase DNA Fragments by Short Read Lengths Review Article
Pages 303-326
Jonathan M. Keith, David B. Hawkes, Jacinta C. Carter, Duncan A.E. Cochran, Peter Adams, Darryn E. Bryant, Keith R. Mitchelson

Chapter 11 Genome Sequencing and Assembly Review Article
Pages 327-355
Annette McGrath

Chapter 12 Valid Recovery of Nucleic Acid Sequence Information from High Contamination Risk Samples – Ancient DNA and Environmental DNA Review Article
Pages 357-371
George A. Kowalchuk, Jeremy J. Austin, Paul S. Gooding, John R. Stephen

Subject Index
Pages 373-381