New high-performance liquid chromatography assay for glycosyltransferases based on derivatization with anthranilic acid and fluorescence detection
โ Scribed by Anumula, K. R.
- Book ID
- 115516351
- Publisher
- Oxford University Press
- Year
- 2012
- Tongue
- English
- Weight
- 136 KB
- Volume
- 22
- Category
- Article
- ISSN
- 0959-6658
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๐ SIMILAR VOLUMES
Three fluorescent derivatization reagents for compounds having hydroxyl and/or amino groups are described. 4-( 2-Phthalimidyl)benzoyl chloride, 3-( 2-phthalimidyl)benzoyl chloride and 3-( 2-phthalimidyl)4-methoxybenzoyl chloride, prepared from the corresponding phthalimidylbenzoic acid, were stable
The enantiomneric separation and the detection of 2-arylpropionic acids after derivatization with the fluorescent reagents with a benzofurazan structure, (S)-( + )-4-(N,N-dimethylaminosulphonyl)-7-(3-aminopyrrolidin-1-yl)-2,1,3-benzoxadiazole ((S)-DBD-Apy), (R)-(-)-4-nitro-7-(3-aminopyrrolidin-1-yl)
A simple and highly sensitive method based on the derivatization of aliphatic amines with 2- (2-phenyl-1H-phenanthro-[9,10-d]imidazole-1-yl)-acetic acid (PPIA) followed by high-performance liquid chromatography (HPLC) with fluorescence detection and on-line post-column mass spectrometric identificat