New genome type of adenovirus serotype 19 causing nosocomial infections of epidemic keratoconjunctivitis in Japan
✍ Scribed by Keico Tanaka-Yokogui; Norihiko Itoh; Norio Usui; Satoshi Takeuchi; Eiichi Uchio; Koki Aoki; Masahiko Usui; Shigeaki Ohno
- Publisher
- John Wiley and Sons
- Year
- 2001
- Tongue
- English
- Weight
- 105 KB
- Volume
- 65
- Category
- Article
- ISSN
- 0146-6615
- DOI
- 10.1002/jmv.2068
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✦ Synopsis
Abstract
Twelve strains of adenovirus serotype 19, isolated from cases of epidemic keratoconjunctivitis in Japan in 1992, 1993, 1997, and 1998, were analyzed by DNA restriction analysis, using restriction endonucleases __Bam__HI, __Bgl__I, __Bgl__II, __Eco__RI, __Hin__dIII, __Kpn__I, __Pst__I, __Sac__I, __Sal__I, __Sma__I, and __Xho__I. Among these 11 restriction endonucleases, __Eco__RI, __Pst__I, __Sac__I, and __Sma__I were discriminative enzymes, showing restriction patterns different from those reported previously for the prototype and the variant 19a. This new genome type was isolated in 1997 and 1998, when an increase of epidemic keratoconjunctivitis cases caused by adenovirus serotype 19 was observed for both sporadic and nosocomial infections. Strains from 1992 and 1993 showed restriction patterns similar to those of the worldwide reported variant 19a for all enzymes used. The changes detected in strains from 1997 and 1998 could be the reason for the recent epidemic. J. Med. Virol. 65:530–533, 2001. © 2001 Wiley‐Liss, Inc.