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New fluorometric assay of lysosomal acid lipase and its application to the diagnosis of wolman and cholesteryl ester storage diseases

✍ Scribed by Anne E. Negre; Robert S. Salvayre; Arie Dagan; Shimon Gatt


Book ID
115825157
Publisher
Elsevier Science
Year
1985
Tongue
English
Weight
528 KB
Volume
149
Category
Article
ISSN
0009-8981

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New spectrophotometric assays of acid li
✍ Anne NΓ¨gre; Robert Salvayre; Arie Dagan; Carla Borrone; Shimon Gatt πŸ“‚ Article πŸ“… 1985 πŸ› Elsevier Science 🌐 English βš– 572 KB

Trinitrophenylaminolauric acid (TNPAL) was linked to glycerol or cholesterol and the resulting yellow compounds were used as substrates for several lipases and cholesteryl esterase in cells from normal individuals and patients with Wolman's or cholesteryl ester storage diseases. Normal cells (lympho