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New enzyme cycling method for determination of oxidized and reduced nicotinamide-adenine dinucleotide

✍ Scribed by Charles L. Woodley; Naba K. Gupta


Publisher
Elsevier Science
Year
1971
Tongue
English
Weight
401 KB
Volume
43
Category
Article
ISSN
0003-2697

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(4R)-Deuterated-reduced nicotinamide adenine dinucleotide phosphate, \((4 R)-\left[{ }^{2} H\right] N A D P H\), was prepared by reduction of NADP \({ }^{+}\)using an NADP \({ }^{+}\)-dependent alcohol dehydrogenase (EC 1.1 .1 .2 ) from Thermoanaerobium brockii and isopropanol- \(d_{8}\) as substrat