New electrocatalytic biomolecular interface for fabricating a fructose dehydrogenase-based sensing system
✍ Scribed by Anwara Begum; Eiry Kobatake; Toshiyuki Suzawa; Yoshihito Ikariyama; Masuo Aizawa
- Publisher
- Elsevier Science
- Year
- 1993
- Tongue
- English
- Weight
- 577 KB
- Volume
- 280
- Category
- Article
- ISSN
- 0003-2670
No coin nor oath required. For personal study only.
✦ Synopsis
An enzyme electrode was fabricated by coupling a pyrroloquinoline quinone (PQQ)containing oxidoreductase with a novel electrode material, 'ITF-TCNQ conducting salt in a polypyrrole matrix. The enzyme electrode was characterized by direct electron transfer between the reduced prosthetic moiety (PQQH,) and the transducing material at a less extreme potential due to the electrocatalytic function of the organic conducting salt. The oxidation of the reduced enzyme occured at 0.2 V vs. Ag/AgCl. The combination of the organic conducting salt and the conductive polymer exhibited an effective molecular interface where direct electron transfer between the PQQ enzyme and the transducing electrode proceeded at a less extreme potential. Here the conductive polymer matrix played important roles, i.e., facilitation of smooth electronic communication and matrix of enzyme immobilization. On the other hand, 'ITF-TCNQ played another role, i.e., electrocatalysis in the regeneration of fructose dehydrogenase. The resulting amperometric fructose sensor is promising, as it was operated at a less extreme potential. &wor& Biosensors; Catalytic methods; Enzymatic methods; Fructose sensor; Pyrroloquinoline quinone
Advances in bioelectrochemistry have permit-prosthetic groups of redox enzymes and irreted the use of a variety of strategies toward the construction of bioelectronic devices in which electronic communication between electrode materials and enzymes plays an important role [l-8]. Biomolecules, especially the enzymes capable of catalyzing redox reactions (oxidoreductases), are now extensively used for the fabrication of bioelectronic devices such as amperometric biosensors. However, direct electron transfer is seldom achieved due to the deep location of active-site