New developments in isotope labeling strategies for protein solution NMR spectroscopy
β Scribed by Natalie K Goto; Lewis E Kay
- Book ID
- 114314330
- Publisher
- Elsevier Science
- Year
- 2000
- Tongue
- English
- Weight
- 195 KB
- Volume
- 10
- Category
- Article
- ISSN
- 0959-440X
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
Nuclear magnetic resonance is an important tool for high-resolution structural studies of proteins. It demands high protein concentration and high purity; however, the expression of proteins at high levels often leads to protein aggregation and the protein purification step can correspond to a high
## Abstract Paramagnetic metal ions bound to proteins generate a dipolar field that can be accurately probed by pseudocontact shifts (PCS) displayed by the proteinβs nuclear spins. PCS are highly useful for determining the coordinates of individual spins in the molecule and for rapid structure dete