New broad-host-range promoter probe vectors based on the plasmid RK2 replicon
β Scribed by Pedro Miguel Santos; Ilaria Di Bartolo; Janet Martha Blatny; Elisabetta Zennaro; Svein Valla
- Book ID
- 117516714
- Publisher
- John Wiley and Sons
- Year
- 2001
- Tongue
- English
- Weight
- 202 KB
- Volume
- 195
- Category
- Article
- ISSN
- 0378-1097
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By coupling the PmΓxylS promoter system to minimal replicons of the broad-host-range plasmid RK2 we recently showed that such vectors are useful for both high-and low-level inducible expression of cloned genes in gram-negative bacteria. In this report, we extend this potential by identifying point m
The cleavage map of the plasmid RK2 was determined for the five restriction endonucleases EcoRI, HindIII, BamH-I, SalI and HpaI. DNA has been inserted into several of these sites and cloned in Escherichia coli. Efforts to obtain derivatives of RK2 reduced in size by restriction endonuclease digestio