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New Aspects of the Plasma Carrier Protein for 25-Hydroxycholecalciferol in Vertebrates

โœ Scribed by H. VAN BAELEN; K. ALLEWAERT AND; R. BOUILLON

Book ID
119862243
Publisher
John Wiley and Sons
Year
1988
Tongue
English
Weight
416 KB
Volume
538
Category
Article
ISSN
0890-6564

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โœ K.T. Koshy; A.L. VanDerSlik ๐Ÿ“‚ Article ๐Ÿ“… 1976 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 496 KB

A high-pressure liquid chromatographic (hplc) procedure was developed for the determination of 2%hydroxycholecalciferol(25-OH-DJ in cow plasma or serum. The procedure involved extraction with an ethanol-ethyl ether mixture, separation of the aqueous phase, solvent partitions, column chromatography o

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In a previous paper [(1976) Anal. Biochem. 74, 2821 we reported a procedure for the analysis of 25hydroxycholecalciferol (25OH-Da) in cow plasma or serum by high performance liquid chromatography. Since then, we obtained synthetic 25hydroxyergocalciferol (25OH-D& and found that it had the same reten