✦ LIBER ✦

New amperometric dehydrogenase electrodes based on electrocatalytic NADH-oxidation at poly (methylene blue)-modified electrodes

✍ Scribed by Arkady A. Karyakin; Elena E. Karyakina; Wolfgang Schuhmann; Hanns-Ludwig Schmidt; Sergei D. Varfolomeyev

Publisher: John Wiley and Sons
Year: 1994
Tongue: English
Weight: 873 KB
Volume: 6
Category: Article
ISSN: 1040-0397
DOI: 10.1002/elan.1140061003

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✦ Synopsis

Poly(methy1ene blue) (PMBI-modified electrodes show excellent properties for the electrocatal>ric oxidation of NADH concerning the optimal working potential of 0 to 100 my (vs. SCE) and their operation stability. By means of deh!.drogenases entrapped within a Nafion film on top of the PMB layer. new amperometric biosensors for glucose, lactate, malate, and ethanol could be obtained with improved integration of the enzyme and the electrocatalyst directly on the electrode surface. Coentrapment of the negatively charged NAD+ within the negatively charged Nafion polyelectrolye leads to reagentless dehydrogenase electrodes for one-shot or short-term applications.

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