New amino-nitroxide spin labels

✍ Scribed by Ileana Dragutan; Agneta Caragheorgheopol; Filip Chiraleu; Rolf J. Mehlhorn

• Book ID: 103992350
• Publisher: Elsevier Science
• Year: 1996
• Tongue: English
• Weight: 624 KB
• Volume: 4
• Category: Article
• ISSN: 0968-0896
• DOI: 10.1016/0968-0896(96)00150-2

✦ Synopsis

Stable free mono-and diradicals containing reactive primary or secondary amino groups in the side-chain have been synthesized by transesterification of amino-substituted esters with paramagnetic alcohols or from spinqabeled acid derivatives and amines. In the second approach the new radical 18 (1-oxyl-3-(2-bromoethoxycarbonyl)-2,2,5,5-tetramethylpyrroline) is proposed as an efficient alkylating species. The nitroxides described are pH-sensitive spin probes and spin labels potentially useful for a diversity of ESR applications in chemistry and biology. New spin-labeled tyramine 16 (N-(1-oxyl-3-carbonyl-2,2,5,5-tetramethylpyrroline)tyramine) was successfully employed in a novel assay of protein oxidative damage.