Neutralization of macrophage inflammatory protein-2 blocks the febrile response induced by lipopolysaccharide in rats

✍ Scribed by Eva Tavares; Maria L. Ojeda; Rosario Maldonado; Francisco J. Miñano

• Publisher: Elsevier Science
• Year: 2004
• Tongue: English
• Weight: 263 KB
• Volume: 29
• Category: Article
• ISSN: 0306-4565
• DOI: 10.1016/j.jtherbio.2004.08.061

✦ Synopsis

1. This study was aimed to test the hypothesis that macrophage inflammatory protein-2 (MIP-2), a powerful chemotactic cytokine for neutrophils, plays a role in bacterial endotoxin fever.

2. The effect of specific anti-rat MIP-2 antibodies on lipopolysaccharide (LPS)-induced fever was tested. Intraperitoneal injection of LPS resulted in a biphasic fever and a significant increase in serum MIP-2 and prostaglandin (PG) E 2 levels which correlated with the start of fever. Intraperitoneal anti-MIP-2 (500 mg/kg) did not affect the body core temperature of unrestrained rats, but markedly attenuated LPS-induced fever.

3. Treatment with the cyclooxygenase inhibitor ibuprofen (10 mg/kg) resulted in a significant attenuation of LPSinduced fever and a significant decrease of MIP-2 and PGE 2 production.

4. These results indicate that LPS fever in rats is, at least, in part dependent on mechanisms involving neutrophils chemotaxis, and that MIP-2 may be an important mediator in the genesis of fever via prostaglandin-dependent pathways. r Published by Elsevier Ltd.