Neutralization of growth-inhibitory material present in calf serum by conditioning factors elaborated by chick embryo cells in culture

Decreased heme content and cessation of

Decreased heme content and cessation of cell growth in cultured chick embryo fibroblasts in the presence of horse serum: Stimulation of heme synthesis and cell growth by iron

✍ Claude Verger; José Imbenotte 📂 Article 📅 1982 🏛 John Wiley and Sons 🌐 English ⚖ 586 KB

## Abstract A new spectrofluorometric method for heme quantitation in cultured fibroblasts is described. The method includes: (1) heme extraction by methanol/sulfuric acid, (2) partial purification of heme by a microchromatographic method, and (3) treatment of the purified heme by oxalic acid follo