NEUTRAL ENDOPEPTIDASE-24.11 (NEP) DEACTIVATES PDGF- AND TGF-β-INDUCED CELL SHAPE CHANGES IN INVERTEBRATE IMMUNOCYTES
✍ Scribed by Eva Caselgrandi; Dimitris Kletsas; Enzo Ottaviani
- Book ID
- 102568393
- Publisher
- Elsevier Science
- Year
- 2000
- Tongue
- English
- Weight
- 157 KB
- Volume
- 24
- Category
- Article
- ISSN
- 1065-6995
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✦ Synopsis
Abstract
Using a cytofluorimetric assay, we found that immunocytes of the mollusc, Mytilus galloprovincialis, express CD10, a surface antigen known to be identical to neutral endopeptidase‐24.11 (NEP). The spectrofluorimetric analysis demonstrates that the growth factors PDGF‐AB and TGF‐β1 provoke an increase in NEP‐like activity in membrane preparations from the immunocytes, but have no effect on the soluble form in the serum. On the other hand, computer‐assisted microscopic image analysis reveals that NEP deactivates the PDGF‐AB‐ and TGF‐β1‐induced shape changes in immunocytes. However, Western blots show that, in solution, NEP does not cleave PDGF‐AB or TGF‐β1, indicating that the inactivation is not due to proteolysis. These results suggest a functional interplay in invertebrate immunocytes between growth factors and NEP, as previously shown in vertebrate cells.