𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Neurotrophin receptor p75NTR suppresses growth and nerve growth factor-mediated metastasis of human prostate cancer cells

✍ Scribed by Scott Krygier; Daniel Djakiew

Publisher
John Wiley and Sons
Year
2002
Tongue
French
Weight
440 KB
Volume
98
Category
Article
ISSN
0020-7136

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

The loss of tumor‐ and/or metastasis‐suppressor gene function contributes to the transformation of human prostate epithelial cells to a malignant pathology. Such a putative tumor‐suppressor and metastasis‐suppressor gene(s) has been mapped to the region of 17q21, which coincidentally is in the vicinity of the human gene locus for the neurotrophin receptor p75^NTR^. The p75^NTR^ is expressed in normal human prostate epithelial cells and exhibits an inverse association of p75^NTR^ expression with the malignant progression of the prostate, consistent with a pathologic role of the p75^NTR^ as a putative tumor and metastasis suppressor. Utilizing stable transfectants of the TSU‐pr1 and PC‐3 human prostate tumor cell lines that exhibit a rank order (dose‐dependent) increase in p75^NTR^ protein expression, we investigated the effects of the p75^NTR^ in combination with its predominant ligand, nerve growth factor (NGF), on tumor cell growth. A rank order (dose‐dependent) increase in p75^NTR^ expression was found to suppress the growth of prostate tumors in severe combined immunodeficient (SCID) mice. Treatment of these tumors with NGF stimulated both proliferation as indicated by PCNA expression and apoptosis as indicated by TUNEL assay, the net result of which was no change in the overall growth of the tumors. However, NGF was found to increase the formation of satellite tumors, both contiguous and noncontiguous with respect to the primary tumor mass, indicating dose‐dependent induction of metastasis. Significantly, the formation of satellite tumors was suppressed by the expression of p75^NTR^. This suggests that p75^NTR^ is a tumor suppressor of growth and a metastasis suppressor of NGF‐stimulated migration of human prostate tumor cells. © 2001 Wiley‐Liss, Inc.

📜 SIMILAR VOLUMES

Expression of p75NTR in a human prostate
Expression of p75NTR in a human prostate epithelial tumor cell line reduces nerve growth factor–induced cell growth by activation of programmed cell death
✍ Beth Pflug; Daniel Djakiew 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 262 KB 👁 2 views

Epithelial expression of the 75-kDa low-affinity neurotrophin receptor (p75 NTR ) is inversely associated with the malignant progression of the human prostate. To elucidate the function of p75 NTR in the prostate, the human prostate epithelial tumor cell line TSU-pr1, which does not express p75 NTR

Regulation of nerve growth factor and it
Regulation of nerve growth factor and its low-affinity receptor (p75NTR) during myogenic differentiation
✍ Christian Erck; Christof Meisinger; Claudia Grothe; Klaus Seidl 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 294 KB 👁 2 views

In our preceding report, we have shown that nerve growth factor (NGF) and its low-affinity receptor (p75 NTR ) are expressed in C2C12 myoblasts and downregulated during myogenic differentiation. Furthermore, NGF affects myogenic differentiation and cell growth via p75 NTR and downregulation of p75 N

Growth arrest of PC12 cells by nerve gro
Growth arrest of PC12 cells by nerve growth factor is dependent on the phosphatidylinositol 3-kinase/Akt pathway via p75 neurotrophin receptor
✍ Hisanori Ito; Hiroshi Nomoto; Shoei Furukawa 📂 Article 📅 2003 🏛 John Wiley and Sons 🌐 English ⚖ 127 KB

## Abstract We recently isolated mutant PC12 cell clones (PC84 cells) by transfection of PC12 cells with nerve growth factor (NGF) cDNA. These cells secreted active NGF and extended short processes, but proliferated faster than the parental PC12 cells. Because the expression level of p75, a low‐aff

Characterization of nerve growth factor
Characterization of nerve growth factor precursor protein expression by human prostate stromal cells: A role in selective neurotrophin stimulation of prostate epithelial cell growth
✍ Delsite, Robert; Djakiew, Daniel 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 412 KB 👁 2 views

## BACKGROUND. Nerve growth factor (NGF) immunoreactive proteins derived from human prostatic stromal cells (hPS) have been implicated in the paracrine regulation of prostate epithelial cell growth. However, mature NGF␤ does not appear to be expressed by these cells. In order to determine whether N

Evidence for the participation of nerve
Evidence for the participation of nerve growth factor and its low-affinity receptor (p75NTR) in the regulation of the myogenic program
✍ Klaus Seidl; Christian Erck; Astrid Buchberger 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 530 KB

We have studied expression and function of neurotrophins and their receptors during myogenic differentiation of C2C12 cells, a clonal cell line derived from mouse muscle that is capable of in vitro differentiation. The genes coding for nerve growth factor (NGF), brain-derived neurotrophic factor (BD

Loss of low-affinity nerve growth factor
Loss of low-affinity nerve growth factor receptor during malignant transformation of the human prostate
✍ Perez, Manuel; Regan, Terry; Pflug, Beth; Lynch, John; Djakiew, Daniel 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 429 KB 👁 2 views

## Background: The low-affinity nerve growth factor receptor (lngfr) exhibits an inverse association of epithelial expression with the degree of differentiation of prostate adenocarcinoma tissue. however, the stage at which loss of lngfr expression is first manifested in the malignant prostate has