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Neurotoxic activity of Gln49 phospholipase A2 from Gloydius ussuriensis snake venom

✍ Scribed by Yan Chang; Yingxue Li; Yongming Bao; Lijia An

Publisher
John Wiley and Sons
Year
2007
Tongue
English
Weight
298 KB
Volume
27
Category
Article
ISSN
0260-437X

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✦ Synopsis

A novel neurotoxic protein phospholipase A 2 (PLA 2 ), molecular weight 13 881.83 Da, has been isolated from snake venom of Gloydius ussuriensis, named as Gln49-PLA 2 , which shows weak lethal toxic, myotoxic and apparent anticoagulant activity, but lacks phospholipase activity. The Gln49-PLA 2 obviously induced an increase of the pain threshold in intoxicated 615 mice compared with the control group, suggesting it is a neurotoxin. Hot-plate tests also showed that its analgesic activity was dose-dependent, and naloxone antagonized the analgesic effect, implying the mechanism of action of Gln49-sPLA 2 is correlated with opioid receptors. Electrophysiology studies revealed decreases in the action potential and the nerve conduction velocity in isolated hoptoad (Bufo bufo gargarizans Cantor) sciatic nerve, indicating Gln49-PLA 2 most probably had effects on ion channels.

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