Neuropeptide Protocols Volume 73 || Purification of Extracted Peptides for Structural Analysis

✍ Scribed by Irvine, G. Brent; Williams, Carvell H.

• Book ID: 120276857
• Publisher: Humana Press
• Year: 1996
• Tongue: English
• Weight: 793 KB
• Edition: 1997
• Category: Article
• ISBN-13: 9780896033993
• DOI: 10.1385/0-89603-399-6:9

✦ Synopsis

Preparation Of Neuropeptide-containing Fractions From Biological Materials / J. Michael Conlon -- Purification Of Extracted Peptides For Structural Analysis / Chris Shaw -- Amino Acid Sequencing Of Neuropeptides / Ka Wan Li, Wijnand P. Geraerts -- Neuropeptide Gene Identification Using The Polymerase Chain Reaction / Aaron G. Maule, Timothy G. Geary -- Solid-phase Synthesis Of Neuropeptides By Fmoc Strategies / Chris Kowalczyk, Michael O'shea -- Incorporation Of Stable Pseudopeptide Bonds : Methylene Amino, Thioether, And Hydroxyethylene Derivatives / Graeme J. Anderson -- Synthesis Of Conformationally Restricted Peptides / Annette G. Beck-sickinger -- Purification Of Synthetic Peptides By High Performance Liquid Chromatography / D. David Smith, Ann M. Hanly -- Molecular Weight Estimation For Neuropeptides Using Size-exclusion High Performance Liquid Chromatography / G. Brent Irvine --^ Molecular Weight Determinations Using Polyacrylamide Gel Electrophoresis With Tris-tricine Buffers / G. Brian Wisdom -- Determination Of Neuropeptides By Capillary Electrophoresis / Sungae S. Park, Wei-lun Hung, Daniel E. Schaufelberger, Norberto A. Guzman, Juan P. Advis -- Characterization Of Neuropeptide Processing By Fast Atom Bombardment Mass Spectrometry / Jerzy Silberring -- Analysis Of Neuropeptides By Size-exclusion Hplc Linked To Electrospray Ionization Mass Spectrometry / Jerzy Silberring -- Identification Of Peptides By Matrix-assisted Laser Desorption Ionization Time Of Flight Mass Spectrometry (maldi-tof-ms) And Direct Analysis Of The Laterobuccal Nerve From The Pond Snail Lymnaea Stagnalis / Glenn Critchley, Belinda Worster -- Use Of Circular Dichroism To Determine Secondary Structure Of Neuropeptides / Laszlo Otvos -- ¹h Nuclear Magnetic Resonance (nmr) In The Elucidation Of Peptide Structure / David J. Guthrie --^ The Study Of Membrane- Or Receptor-bound Neuropeptides By Nmr / Rickey P. Hicks -- Molecular Modeling Of Neuropeptides / Sándor Lovas, Richard F. Murphy -- Tritium Labeling Of Neuropeptides / Géza Tóth, Sándor Lovas, Ferenc Ötvös -- The Use Of Iodo-gen For Preparing ¹²⁵i-labeled Peptides And Their Purification By Reversed-phase High Performance Liquid Chromatography / J. Michael Conlon -- Production Of Antisera Using Peptide Conjugates / Thomas E. Adrian -- Radioimmunoassay / Thomas E. Adrian -- Enzyme-linked Immunosorbent Assay Of Peptides / Laszlo Otvos, Gyorgyi I. Szendrei -- Sample Preparation For Peptide Immunocytochemistry / Kathy M. Pogue, Colin F. Johnston -- Immunocytochemical Methods For Regulatory Peptides / Kathy M. Pogue, Colin F. Johnston -- Ultrastructural Localization Of Peptides Using Immunogold Labeling / David W. Halton, Gerard P. Brennan --^ Preparation Of A Membrane Fraction For Receptor Studies And Solubilization Of Receptor Proteins With Retention Of Biological Activity / Mark Wheatley, John Howl, Nicola J. Yarwood, Andrew R. Davies, Rosemary A. Parslow -- Radioligand Binding Using ¹²⁵i-labeled Peptides / Peter W. Abel, David Waugh, William B. Jeffries -- Analysis Of Data From Cold Saturation Radioligand Binding Experiments / William B. Jeffries, David Waugh, Peter W. Abel -- Organ/tissue Preparations For The Assessment Of Agonist/antagonist Activity / Mark Murnin, Sándor Lovas, James M. Allen, Richard F. Murphy -- Measurement Of Efflux Rates From Brain To Blood / William A. Banks, Melita B. Fasold, Abba J. Kastin -- Assay Of Neuropeptidases Using Fluorogenic Substrates / Carvell H. Williams -- Characterization Of Neuropeptidases Using Inhibitors / Nigel M. Hooper. Edited By G. Brent Irvine, Carvell H. Williams. Includes Bibilographical References And Index.