Neuronal precursors of the adult rat subependymal zone persist into senescence, with no decline in spatial extent or response to BDNF
✍ Scribed by Goldman, Steven A. ;Kirschenbaum, Barry ;Harrison-Restelli, Catherine ;Thaler, Howard T.
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- English
- Weight
- 553 KB
- Volume
- 32
- Category
- Article
- ISSN
- 0022-3034
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✦ Synopsis
The adult mammalian brain continin the 3-month-old rats compared with their aged ues to harbor ependymal/subependymal zone (SZ) counterparts, but neuronal outgrowth was robust at precursor cells, which can give rise to neurons in vitro.
each age tested. The duration of survival of SZ-de-In adult rats, explants of the rostral 6-7 mm of the rived neurons did not differ between the young and SZ give rise to neurons in vitro, and over this entire old rats. At both ages, BDNF supported the survival expanse, neuronal survival is supported specifically by of these new adult neurons. The extent of BDNF's brain-derived neurotrophic factor (BDNF). We asked influence was independent of both the age of the donor whether either the (a) spatial distribution, (b) abunrat and the rostrocaudal level at which the parent SZ dance, or (c) BDNF responsiveness of the neuronal explant was taken. Thus, the neuronal precursors of precursor population was affected by age. Explants of the rat brain persist into senescence; the size of the three rostrocaudally defined regions were taken from precursor pool attenuates minimally with age, and its both young and old rats (3 and 20 months old, respecspatial extent remains constant. The neurons genertively), and cultured in 2% fetal bovine serum-conated from these precursors can respond to BDNF taining media with or without added BDNF (20 ng/ throughout life. ᭧ 1997 John Wiley & Sons, Inc. J Neurobiol mL). The extent of neuronal production by these ex-32: 554-566, 1997 plants varied only minimally with their level of deriva-Keywords: ventricular zone; stem cells; aging; neurotion, such that substantial outgrowth was observed at trophins; adult neurogenesis each level tested. Neuronal outgrowth was marginally rise to neurons both in vitro and in vivo.