Neuromodulatory actions of dopamine on synaptically-evoked neostriatal responses in slices
✍ Scribed by Michael S. Levine; Zhiwei Li; Carlos Cepeda; Howard C. Cromwell; Katharine L. Altemus
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- English
- Weight
- 606 KB
- Volume
- 24
- Category
- Article
- ISSN
- 0887-4476
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✦ Synopsis
7-OH-DPAT has been shown to exhibit selectivity between Dz and D3 dopamine receptors in a variety of in vitro assay systems. Although the drug has been used in a variety of studies to evaluate the behavioral effects of D3 receptor stimulation, the in vivo Dz/D3 selectivity of the compound has not been determined. In this study, protection against inactivation by EEDQ was used as a measure of in vivo occupancy of D2 receptors by behaviorally relevant doses of 7-OH-DPAT (0.03-10 mg/kg, s.c.) in adult, male Sprague-Dawley rats. Ex vivo [3Hlspiperone binding was then determined in striatal membranes. 7-OH-DPAT protected against inactivation of Dz receptors with an EDSo value of 11 mg/kg. Significant protection of Dz receptors was observed at doses of 1 mgkg and above. These data suggest that stimulation of Dz receptors contributes to the pharmacological effects of 7-OH-DPAT when administered at doses above 0.3 mg/ kg (s.c.). o 1996 Wiley-Liss, Inc.