✦ LIBER ✦

Neural cell adhesion molecule-mediated neurite outgrowth is repressed by overexpression of HES-1

✍ Scribed by Ulla Jessen; Vera Novitskaya; Peter S. Walmod; Vladimir Berezin; Elisabeth Bock

Publisher: John Wiley and Sons
Year: 2002
Tongue: English
Weight: 170 KB
Volume: 71
Category: Article
ISSN: 0360-4012
DOI: 10.1002/jnr.10433

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

The neural cell adhesion molecule (NCAM) stimulates neurite outgrowth by activating intracellular signaling cascades. We investigated the role of the transcriptional repressor HES‐1 in NCAM‐dependent neurite outgrowth by estimating neurite extension from PC12‐E2 cells grown in coculture with NCAM‐negative or NCAM‐positive fibroblasts. PC12‐E2 cells were transiently transfected with an expression plasmid encoding HES‐1. We found that expression of HES‐1 inhibited NCAM‐dependent neurite outgrowth. Treatment with arachidonic acid (an important messenger in NCAM‐dependent signaling) restored NCAM‐induced neurite outgrowth inhibited by HES‐1. These results suggest that HES‐1 is a regulator of intracellular signal transduction stimulated by cell adhesion molecules involved in neurite outgrowth. © 2002 Wiley‐Liss, Inc.

📜 SIMILAR VOLUMES

Missense mutations in the extracellular

Missense mutations in the extracellular domain of the human neural cell adhesion molecule L1 reduce neurite outgrowth of murine cerebellar neurons

✍ Piret Michelson; Christine Hartwig; Melitta Schachner; Andreas Gal; Andres Veske 📂 Article 📅 2002 🏛 John Wiley and Sons 🌐 English ⚖ 322 KB

Mutations in L1CAM, the gene encoding the transmembrane multifunctional neuronal adhesion molecule L1, are associated with neurodevelopmental disorders including X-linked hydrocephalus and mental retardation. Some amino acid substitutions in various extracellular domains of L1 are known to affect po