Two major variables may cause false-negative results in prenatal diagnosis of congenital human cytomegalovirus (HCMV) infection: sensitivity of the technique(s) used; and time elapsed between maternal infection and antenatal testing. Previous results indicated that rapid HCMV isolation from amniotic
Nested polymerase chain reaction in the diagnosis of congenital cytomegalovirus infection
β Scribed by R. Sivakumar; Niti Singh; Sarman Singh
- Book ID
- 110652937
- Publisher
- Springer-Verlag
- Year
- 2001
- Tongue
- English
- Weight
- 684 KB
- Volume
- 68
- Category
- Article
- ISSN
- 0019-5456
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## Abstract The reliability of the polymerase chain reaction (PCR) for prenatal diagnosis of human cytomegalovirus (HCMV) infection was determined by retrospective testing of 35 amniotic fluids identified previously as positive or negative for HCMV by virus isolation. Amniocentesis was performed in
Human cytomegalovirus (HCMV) is the most common cause of viral intra-uterine infection. The experience with prenatal diagnosis remains limited and is based on few reports of small numbers of cases. It is thus difficult to compare the accuracy of the different tests because the groups studied were sm