## Abstract A highly sensitive twoβstep polymerase chain reaction (PCR) method was evaluated for detection of human immunodeficiency virus type 1 (HIVβ1) DNA in clinical specimens. The product resulting from the first amplification reaction is used as the template for the second PCR with an interna
β¦ LIBER β¦
Nested polymerase chain reaction for detection of Ehrlichia equi genomic DNA in horses and ticks (Ixodes pacificus)
β Scribed by Jeffrey E. Barlough; John E. Madigan; Elfriede DeRock; Luisa Bigornia
- Publisher
- Elsevier Science
- Year
- 1996
- Tongue
- English
- Weight
- 612 KB
- Volume
- 63
- Category
- Article
- ISSN
- 0304-4017
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## Abstract Persistent B19 parvovirus infection has been recognized in immunocompromised patients, often occurring with a lowβtiter viremia. In this study, nested polymerase chain reaction (PCR) for the detection of B19 parvovirus DNA was carried out on the sera of 49 human immunodeficiency virus (