Nerve growth factor: Subunit interactions in the mouse submaxillary gland 7S complex

✍ Scribed by Robert E. Silverman; Ralph A. Bradshaw

• Book ID: 102908313
• Publisher: John Wiley and Sons
• Year: 1982
• Tongue: English
• Weight: 562 KB
• Volume: 8
• Category: Article
• ISSN: 0360-4012
• DOI: 10.1002/jnr.490080204

✦ Synopsis

Abstract

Sedimentation velocity analyses of the isolated subunits of mouse 7S nerve growth factor (NGF) and their binary complexes have been performed. The 2.5S (β) form of NGF binds 2 α subunits independent of the presence of γ subunits. Two γ subunits are also complexed by 2.5S NGF; however, this interaction is dependent on the presence of a carboxyl terminal arginine residue on the β polypeptide chain. The partial loss of this residue in 2.5S NGF preparations results in a ternary complex(es) with a reduced sedimentation coefficient(s). The two binary complexes formed (α~2~‐β and β‐γ~2~) show markedly different pH stability profiles. The α and γ subunits do not form a demonstrable complex, but apparently can interact in the ternary complex to render it more stable than the sum of the α‐β and β‐γ interactions.