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Nerve growth factor immunoreactivity in mouse kidney: An immunoelectron microscopic study

✍ Scribed by L. Barajas; E. C. Salido; N. P. Laborde; D. A. Fisher

Publisher: John Wiley and Sons
Year: 1987
Tongue: English
Weight: 622 KB
Volume: 18
Category: Article
ISSN: 0360-4012
DOI: 10.1002/jnr.490180306

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✦ Synopsis

We have recently reported the immunolocalization of nerve growth factor (NGF) in mouse kidney by light microscopy. In the present study, we have investigated the ultrastructural localization of NGF by the preembedding immunoperoxidase method for electron microscopy. NGF immunoreactivity was present in the connecting tubule cells of the distal nephron. These cells showed immunostaining associated with the Golgi complex, vesicles, rough endoplasmic reticulum (RER), and polyribosomes. The intercalated cells, in contrast, lacked immunoreactivity.

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