Nereis virens (Annelida: Polychaeta) is not an adequate sentinel species to assess the genotoxic risk (comet assay) of PAH exposure to the environment
✍ Scribed by Marlies De Boeck; Micheline Kirsch-Volders
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- English
- Weight
- 132 KB
- Volume
- 30
- Category
- Article
- ISSN
- 0893-6692
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✦ Synopsis
Polychaetes, because of their bioturbation capacity, For each treatment four animals were analysed. play an important role in the distribution of anthro-After 1 hr treatment coelomocytes were harvested pogenic contaminants (including polycyclic aro-by puncturing the coelomic cavity with a sharpened matic hydrocarbons [PAHs]) throughout the sedi-Pasteur pipette, mixed with 0.5% low melting point ments. In this work the use of Nereis virens (Anne-agarose and sandwiched between two other gel lida: Polychaeta) as a bioindicator to assess the layers. Ethidium bromide stained nuclei were anagenotoxic risk of PAH exposure for the environment lysed for tail length and tail moment. 12.1 mg/ml was evaluated. For this purpose the alkaline single EMS, pure DMSO (98.9%) and B[a]P in all tested cell gel electrophoresis (comet) assay was applied concentrations induced a statistically significant inon the coelomocytes of in vivo exposed Nereis vircrease of DNA single strand breaks in the comet ens. Benzo[a]pyrene (B[a]P) was chosen because it assay. The effect of B[a]P, however, was only at is classified by the IARC (International Agency for the highest concentration (45 mg/ml) significantly Research on Cancer) as ''probably carcinogenic to stronger than the effect of DMSO alone. Although humans'' and because its mechanisms of action are a relatively large heterogeneity in the results could well-known. Nereis virens was exposed to B[a]P in be observed, these experiments clearly showed that concentrations of 0.3, 0.6, 10, 20, 35 and 45 mg/ Nereis virens is not suited as a sentinel species for ml by an intracoelomic injection of B[a]P (20 ml) the assessment of the genotoxic risk of PAH expodissolved in dimethyl sulphoxide (DMSO). A solvent sure because this species seems to be very resistant control with DMSO, a positive control with ethyl to benzo[a]pyrene. Environ. Mol. Mutagen. methane sulphonate (EMS) (12.1 mg/ml) and a 30:82-90, 1997 ᭧ 1997 Wiley-Liss, Inc. negative control were included in each experiment.