𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Neovascularization and free microsurgical transfer of in vitro cartilage-engineered constructs

✍ Scribed by Nguyen The Hoang; Christoph Hoehnke; Pham Thu Hien; Veronika Mandlik; Achim Feucht; Rainer Staudenmaier

Publisher
John Wiley and Sons
Year
2009
Tongue
English
Weight
589 KB
Volume
29
Category
Article
ISSN
0738-1085

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Cartilage tissue engineering shows to have tremendous potential for the reconstruction of three‐dimensional cartilage defects. To ensure survival, shape, and function, in vitro cartilage‐engineered constructs must be revascularized. This article presents an effective method for neovascularization and free microsurgical transfer of these in vitro constructs. Twelve female Chinchilla Bastard rabbits were used. Cartilage‐engineered constructs were created by isolating chondrocytes from auricular biopsies, amplifying in monolayer culture, and then seeding them onto polycaprolactone scaffolds. In each prefabricated skin flap, three in vitro cartilage‐engineered constructs (2 × 2 × 0.5 cm) and one construct without cells (served as the control) were implanted beneath an 8 × 15 cm random‐pattern skin flap, neovascularized by implantation of an arteriovenous vascular pedicle with maximal blood flow. Six weeks later, the neovascularized flaps with embedded cartilage‐engineered constructs were completely removed based on the newly implanted vascular pedicle, and then freely retransferred into position using microsurgery. Macroscopic observation, selective microangiography, histology, and immunohistochemistry were performed to determine the construct vitality, neovascularization, and new cartilage formation. The results showed that all neovascularized skin flaps with embedded constructs were successfully free‐transferred as free flaps. The implanted constructs were well integrated and protected within the flap. All constructs were well neovascularized and showed histologically stability in both size and form. Immunohistology showed the existence of cartilage‐like tissue with extracellular matrix neosynthesis. © 2008 Wiley‐Liss, Inc. Microsurgery, 2009.

📜 SIMILAR VOLUMES

In vitro neural injury model for optimiz
In vitro neural injury model for optimization of tissue-engineered constructs
✍ D. Kacy Cullen; Sarah E. Stabenfeldt; Crystal M. Simon; Ciara C. Tate; Michelle 📂 Article 📅 2007 🏛 John Wiley and Sons 🌐 English ⚖ 375 KB

## Abstract Stem cell transplantation is a promising approach for the treatment of traumatic brain injury, although the therapeutic benefits are limited by a high degree of donor cell death. Tissue engineering is a strategy to improve donor cell survival by providing structural and adhesive support

Molecular diffusion in tissue-engineered
Molecular diffusion in tissue-engineered cartilage constructs: Effects of scaffold material, time, and culture conditions
✍ Leddy, Holly A. ;Awad, Hani A. ;Guilak, Farshid 📂 Article 📅 2004 🏛 John Wiley and Sons 🌐 English ⚖ 372 KB

## Abstract Diffusion is likely to be the primary mechanism for macromolecular transport in tissue‐engineered cartilage, and providing an adequate nutrient supply via diffusion may be necessary for cell proliferation and extracellular matrix production. The goal of this study was to measure the dif

Tissue-engineered cartilage of porcine a
Tissue-engineered cartilage of porcine and human origin as in vitro test system in arthritis research
✍ Axel R. Göhring; Carsten Lübke; Kristin Andreas; Christian Kaps; Thomas Häupl; A 📂 Article 📅 2010 🏛 American Institute of Chemical Engineers 🌐 English ⚖ 579 KB

## Abstract The increasing prevalence of cartilage destruction during arthritis has entailed an intensified amount for in vitro cartilage models to analyze pathophysiological processes and to screen for antirheumatic drugs. Tissue engineering offers the opportunity to establish highly organized 3D

Monitoring of lactate and glucose levels
Monitoring of lactate and glucose levels in engineered cartilage construct by microdialysis
✍ O.A. Boubriak; J.P.G. Urban; Z.F. Cui 📂 Article 📅 2006 🏛 Elsevier Science 🌐 English ⚖ 332 KB

In this work, microdialysis is employed to monitor cell metabolism within engineered tissues and the technique is tested with model engineered cartilage. Bovine articular chondrocytes isolated from the metacarpalphalangeal joint were seeded evenly in an alginate gel at the densities ranging from 3.3