Negative ion fast atom bombardment mass spectrometry of oligodeoxynucleotide carbamate analogs
✍ Scribed by D. Griffin; J. Laramée; M. Deinzer; E. Stirchak; D. Weller
- Publisher
- John Wiley and Sons
- Year
- 1988
- Tongue
- English
- Weight
- 494 KB
- Volume
- 17
- Category
- Article
- ISSN
- 1076-5174
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A new series of thymine derivative acyclic nucleosides, with mono-or polyfluorosubstitution on the acyclic portion of the molecules have been studied by fast-atom bombardment mass spectrometry, with the aid of metastable ion studies and deuterium labelling experiments. The protonated molecules show
Negative ion fast atom bombardment mass spectrornetry is used to verify the sequence of ol¡godeoxyri~nucleotides containing a single modified nucleotide. Both the position in the sequence and the rnolecular weight of the modified nucleotide can be determined from the 3 and 5'-phosphate sequence ions