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Needle-in-a-haystack detection and identification of base substitution mutations in human tissues

✍ Scribed by Vincent L Wilson; Qi Wei; Kerry R Wade; Midori Chisa; Deidre Bailey; Christopher M Kanstrup; Xiuqin Yin; Chad M Jackson; Barbara Thompson; William R Lee


Book ID
117768008
Publisher
Elsevier Science
Year
1999
Tongue
English
Weight
466 KB
Volume
406
Category
Article
ISSN
1383-5726

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## Communicated by Richard Wooster Mismatch repair detection (MRD) was used to screen 93 matched tumor-normal sample pairs and 22 cell lines for somatic mutations in 30 cancer relevant genes. Using a starting amount of only 150 ng of genomic DNA, we screened 102 kb of sequence for somatic mutations