Near real-time, on-site, quantitative analysis of PAHs in the aqueous environment using an antibody-based biosensor
✍ Scribed by Candace R. Spier; George G. Vadas; Stephen L. Kaattari; Michael A. Unger
- Publisher
- John Wiley and Sons
- Year
- 2011
- Tongue
- English
- Weight
- 349 KB
- Volume
- 30
- Category
- Article
- ISSN
- 0730-7268
- DOI
- 10.1002/etc.546
No coin nor oath required. For personal study only.
✦ Synopsis
Abstract
Rapid, on‐site, quantitative assessments of dissolved polycyclic aromatic hydrocarbons (PAHs) were demonstrated for two field applications. The platform, a KinExA Inline Sensor (Sapidyne Instruments), employed the monoclonal anti‐PAH antibody, 7B2.3, which has specificity for 3‐ to 5‐ring PAHs. A spatial study was conducted near a dredging site where contaminated sediments were being removed, and a temporal study was performed during a rainfall event. Most importantly, the generation of near real‐time data guided management decisions in the field and determined proper sampling protocols for conventional analyses. The method was able to determine PAH concentrations as low as 0.3 µg/L, within 10 min of sample acquisition, and to assess 80+ samples (not including standards and blanks) in less than 3 d. These results were compared with a laboratory‐based gas chromatography–mass spectrometry method in which a wide array of PAHs, including alkylated homologs, were examined. This system shows great promise as a field instrument for the rapid monitoring of PAH pollution. Environ. Toxicol. Chem. 2011; 30:1557–1563. © 2011 SETAC