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Nature of the interactions involved in the lipid-protein complexes of the Escherichia coliN-acetylmuramoyl-l-alanine amidase

✍ Scribed by Edgard Vanderwinkel; Monique de Vlieghere; Philippe Charles; Véronique Baptist


Book ID
113266677
Publisher
Elsevier Science
Year
1987
Tongue
English
Weight
440 KB
Volume
913
Category
Article
ISSN
0167-4838

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N-acetylmuramoyl-l-alanine amidase assay
✍ Philippe Margot; Claude-Alain H. Roten; Dimitri Karamata 📂 Article 📅 1991 🏛 Elsevier Science 🌐 English ⚖ 468 KB

A sensitive and highly reproducible assay for N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28) was devised, based on specific and homogeneous L-[14C]alanine labeling of the substrate, the peptidoglycan. The method involves partial purification of both the enzyme and the substrate and monitoring the