Nature of permeability changes in membrane of HeLa cells adsorbing sendai virus

✍ Scribed by Pinchas Fuchs; Eva Gruber; Janina Gitelman; Alexander Kohn

• Publisher: John Wiley and Sons
• Year: 1980
• Tongue: English
• Weight: 672 KB
• Volume: 103
• Category: Article
• ISSN: 0021-9541
• DOI: 10.1002/jcp.1041030212

✦ Synopsis

Abstract

Adsorption of Sendai virus to HeLa cells induced in them an increased permeability to K^+^, Na^+^, Ca^++^, deoxyglucose, but not to fluorescein. The stimulation of uptake of ^42^K was temperature‐dependent, did not occur below 15°C, and was not inhibited by ouabain.

The virus‐induced increase in the uptake and release of ^42^K and of ^3^H deoxyglucose could not be mimicked by treatment of cells with linoleic acid, a procedure which increased the fluidity of the cellular membranes. The stimulatory effect of 0.5 mM ATP on the release of deoxyglucose was enhanced several fold in the presence of Sendai virus. These results seem to indicate the possible involvement of membranal enzymes such as e.g. protein kinase in the permeability changes induced by Sendai virus.