Nature of collagen in dermatofibrosarcoma protuberans

✍ Scribed by Lutz Weber; Wilhelm N. Meigel

• Publisher: Springer-Verlag
• Year: 1979
• Tongue: English
• Weight: 972 KB
• Volume: 265
• Category: Article
• ISSN: 0340-3696
• DOI: 10.1007/bf00412701

✦ Synopsis

The nature of collagen from 2 cases of dermatofibrosarcoma protuberans was studied. For this purpose, the tumor tissue was carefully separated from adjacent normal dermis. The collagen types comprised in the tumor were identified by CM-cellulose chromatographic and SDS-gel electrophoretic analysis of the component a-chains. Semiquantitative evaluation of the relative type III content was established by separation of the cyanogen bromide pel~tides on gels of 12 ~o polyacrylamide in SDS. These studies showed that dermatofibrosarcoma protuberans contains el(I)-, e2-, and el(III)-chains as well, and corresponding type I-and type III-related CNBr peptides. Comparing the collagen from dermatofibrosarcoma protuberans to that of normal skin, the relatively increased type III content in the case of dermatofibrosarcoma protuberans becomes apparent.