Natural killer cells and CD56+ T cells in the blood of multiple myeloma patients: Analysis by 4-colour flow cytometry
✍ Scribed by Malcolm A. King; Monica A. Radicchi-Mastroianni
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- English
- Weight
- 419 KB
- Volume
- 26
- Category
- Article
- ISSN
- 0196-4763
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✦ Synopsis
The blood of multiple myeloma patients was examined for non-MHC-restricted cytotoxic lymphocytes. Four colour flow cytometry was used to phenotype the cells within a light scatter gate large enough to include all lymphocytes. NK and T cells were identified using CD16, CD56, and CD3 antibodies, and myeloid cells with CD13 and CD14 antibodies. Three subpopulations of NK cells and 3 subpopulations of CD16+ or CD56+ T cells were enumerated. The CD56+ NK and T cells were also examined with CD69, CD25, and anti-HLA-DR antibodies to assess their activation state. We found no evidence that either the percentage or the absolute number of any subpopulation of the NK cells or CD56+ T cells correlated with disease activity. Neither did we find any significant abnormalities in the numbers of activated CD56+ NK or T cells. We conclude that it is unlikely that circulating non-MHC-restricted cytotoxic lymphocytes are responsible for maintaining disease stability in myeloma patients with indolent disease. o