✦ LIBER ✦

Natural antibodies to HIV-tat epitopes and expression of HIV-1 genes in vivo

✍ Scribed by Willy J. A. Krone; Christine Debouck; Leon G. Epstein; Peter Heutink; Rob Meloen; Dr. Jaap Goudsmit

Publisher: John Wiley and Sons
Year: 1988
Tongue: English
Weight: 628 KB
Volume: 26
Category: Article
ISSN: 0146-6615
DOI: 10.1002/jmv.1890260306

No coin nor oath required. For personal study only.

✦ Synopsis

The tat regulatory protein of HIV-1 was expressed as a fusion protein in E. coli and used as antigen to detect antibodies against HIV-tat (anti-tat) in the serum of HIV-1 infected children and adults. HIV-1-infected children showed a higher frequency (55%) of anti-tat than HIV-1-infected adults (36%). Anti-tat were present in only 15% (3/20) of acutely infected individuals. Forty percent (10/25) of individuals with prolonged HIV-1 infection but without antigen were anti-tat positive. Only 13% (3/23) of HIV-1-antibody-positive individuals with prolonged HIV-1 antigenemia were anti-tat positive and titers of anti-tat antibodies declined with time. Pepscan analysis identified the amino terminus of HIV-tat as the major antibody-binding site. Antibodies to HIV-tat occurred as a harbinger of HIV-1 antigen expression and disappeared thereafter, possibly reflecting the transience of HIV-tat expression. Because of the low antigenicity of HIV-tat, antibodies to this regulatory protein are not a reliable marker for either early HIV-1 infection or subsequent disease progression.

📜 SIMILAR VOLUMES

Regulatory functions of Cdk9 and of cycl

Regulatory functions of Cdk9 and of cyclin T1 in HIV Tat transactivation pathway gene expression

✍ Gaetano Romano; Margaret Kasten; Giulia De Falco; Pietro Micheli; Kamel Khalili; 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 307 KB 👁 2 views

HIV-1 gene expression relies upon a complex machinery that is primarily controlled by two viral regulatory proteins, Tat and Rev. Rev is involved in regulating post-transcriptional events of HIV-1 gene expression. The Tat protein transactivates transcription from the HIV-1 5Ј long terminal repeat (L

Towards developing HIV-2 lentivirus-base

Towards developing HIV-2 lentivirus-based retroviral vectors for gene therapy: Dual gene expression in the context of HIV-2 LTR and Tat

✍ Reza Sadaie, M.; Zamani, Maryam; Whang, Suk; Sistron, Nicki; Arya, Suresh K. 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 712 KB

Because of the distinct ability of retroviruses to integrate into the target cell genome and thus achieve long-term expression, retrovirus vectors hold great promise for stable gene transfer. Such vectors derived from human immunodeficiency retroviruses (HIVs) and other lentiviruses are envisioned t

Permissive residues within the minimal e

Permissive residues within the minimal epitopes of neutralizing monoclonal antibodies to the V3 loop of HIV-1

✍ Isabelle L. Laisney; Howard Benjamin; Malcom Gefter; A. Donny Strosberg 📂 Article 📅 1996 🏛 John Wiley and Sons 🌐 English ⚖ 745 KB

Speed of progression to AIDS and degree

Speed of progression to AIDS and degree of antibody response to accessory gene products of HIV-1

✍ Peter Reiss; Joep M. A. Lange; Anthony de Ronde; Frank de Wolf; John Dekker; Chr 📂 Article 📅 1990 🏛 John Wiley and Sons 🌐 English ⚖ 593 KB

## Abstract Antibodies to __E. coli__‐produced HIV‐1 __nef__, __rev__, __tat__, __vpu__, and __vpr__ proteins were measured by enzyme immunoassay in serial sets of sera from 72 men seroconverting for antibodies to HIV‐1 structural proteins, and from 190 initially symptom‐free men who were seroposit

Increased number of single-LTR HIV-1 DNA

Increased number of single-LTR HIV-1 DNA junctions correlates with HIV-1 antigen expression and CD4+ cell decline in vivo

✍ Suzanne Jurriaans; John Dekker; Marion Cornelissen; Jaap Goudsmit; Anthony de Ro 📂 Article 📅 1995 🏛 John Wiley and Sons 🌐 English ⚖ 827 KB

Human immunodeficiency type 1 (HIV-1) DNA in peripheral blood cells of HIV-1 infected individuals may be present as integrated and/or unintegrated DNA. Several reports have indicated that a major proportion of HIV-1 DNA in the asymptomatic phase is linear, full-length, and unintegrated and in the sy

Involvement of p300 in constitutive and

Involvement of p300 in constitutive and HIV-1 Tat-activated expression of glial fibrillary acidic protein in astrocytes

✍ Wei Zou; Zhenyuan Wang; Ying Liu; Yan Fan; Betty Y. Zhou; X. Frank Yang; Johnny 📂 Article 📅 2010 🏛 John Wiley and Sons 🌐 English ⚖ 856 KB

## Abstract HIV‐1 Tat protein is an important pathogenic factor in HIV‐1‐associated neurological diseases. One hallmark of HIV‐1 infection of the central nervous system (CNS) is astrocytosis, which is characterized by elevated glial fibrillary acidic protein (GFAP) expression in astrocytes. We have