𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Native and mutant nickel–iron hydrogenases: Unravelling structure and function

✍ Scribed by Antonio L. de Lacey; Víctor M. Fernández; Marc Rousset

Publisher
Elsevier Science
Year
2005
Tongue
English
Weight
318 KB
Volume
249
Category
Article
ISSN
0010-8545

No coin nor oath required. For personal study only.

✦ Synopsis

In this review, an appraisal of the progress in understanding of the NiFe-hydrogenases catalytic mechanism is presented. The article is focussed on NiFe-hydrogenases from Desulfovibrio genus, as these are among the best-characterized H 2 -splitting enzymes. The X-ray structures, catalytic reactivity, spectroscopic properties and computer modeling data of the different forms of this class of hydrogenase are analyzed. In addition, the contribution of site-directed mutagenesis studies of Desulfovibrio fructosovorans species to structure-function relationships is reviewed.

📜 SIMILAR VOLUMES

Structural and functional models related
Structural and functional models related to the nickel hydrogenases
✍ Elisabeth Bouwman; Jan Reedijk 📂 Article 📅 2005 🏛 Elsevier Science 🌐 English ⚖ 852 KB

In this review a historic overview is given of the biomimetic models synthesised since the presence of nickel in hydrogenases was first reported in 1981. The crystal structure of the [NiFe] hydrogenase isolated from Desulvofibrio gigas unexpectedly revealed a heterodinuclear active site, including a